Composite

Part:BBa_K2683029:Design

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-10)


Nucleoside Diphosphate Kinase (NDK) in pSB1C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The subparts and coding sequence of this part are optimized for use in E.coli .

Source

https://parts.igem.org/Part:BBa_K2443035:Design

References

[1] Miller, J., Funchain, P., Clendenin, W., Huang, T., Nguyen, A., Wolff, E., Yeung, A., Chiang, J., Garibyan, L., Slupska, M., and Yang, H. (2002) Escherichia coli strains (NDK) lacking nucleoside diphosphate kinase are powerful mutators for base substitutions and frameshift in mismatch-repair-deficient strains. Genetics. 162, 5-13